Therapeutic Potential Of Different Pericyte Populations In The Recovery Of Skeletal Muscle Mass Following Disuse

• DOI: 10.1249/01.mss.0000764688.44536.8d
• Publication Date: 2021-07-12T09:14:31Z
• Authors: Yu-Fu Wu; Samuel Lapp; Svyatoslav Dvoret...; Amanda Tannehill; Gabriela Garcia; Marni D. Boppart
• Keywords: Pericyte; CD146; Hindlimb

Significant loss in skeletal muscle mass and function can occur after periods of extended bed rest or immobilization. Physical therapy is recommended, but recovery may be incomplete special populations due to injury functional limitations. Our lab recently demonstrated the capacity for pericytes, vascular stromal cells, accelerate following disuse a mouse model. Different pericyte exist based on localization unique cell surface markers, yet most therapeutic population has not been identified. PURPOSE: To identify with greatest benefit when transplanted into period disuse. METHODS: Twenty-four 4-month old C57BL/6 mice were randomly divided four groups (n = 6/group). Mice hindlimbs immobilized full dorsiflexion via surgical staple inserted through center foot body gastrocnemius 2 weeks. At weeks post immobilization, staples removed either pericytes (CD146+Lin-, CD146+NG2-Lin-, NG2+Lin-) saline (control) injected tibialis anterior (TA) muscle. TA muscles excised analysis remobilization extent was assessed. One-way ANOVA used compare improvement between treatment groups. RESULTS: No significant change myofiber CSA found one-way ANOVA, noted receiving CD146+Lin-pericytes compared PBS t test (70% recovery; p 0.05). improvements capillarization collagen remodeling detected CD146+NG2-Lin- (101% 244% CHP < 0.05) CD146+Lin- (83% 209% controls. CONCLUSION: transplantation effectively recovered structure controls, whereas NG2+Lin- did demonstrate similar recovery. Supported by NIH Grant NIAMS R01 AR072735 MDB.