Antioxidant Supplementation Reduces Skeletal Muscle Mitochondrial Biogenesis

• DOI: 10.1249/mss.0b013e318203afa3
• Publication Date: 2010-11-16T10:44:59Z
• Authors: NATALIE A. STROBEL; JONATHAN M. PEAKE; AYA MATSUMOTO; SUSAN A. MARSH; JEFF S. COOMBES; GLENN D. WADLEY
• Keywords: SOD2; Endurance Training

Exercise increases the production of reactive oxygen species (ROS) in skeletal muscle, and athletes often consume antioxidant supplements belief they will attenuate ROS-related muscle damage fatigue during exercise. However, exercise-induced ROS may regulate beneficial adaptations, such as increased mitochondrial biogenesis. We therefore investigated effects long-term supplementation with vitamin E α-lipoic acid on changes markers biogenesis exercise-trained sedentary rats.Male Wistar rats were divided into four groups: 1) control diet, 2) 3) exercise 4) diet. Animals ran a treadmill 4 d · wk at ∼ 70%VO2max for up to 90 min 14 wk.Consistent augmentation defenses, after training there significant peroxisome proliferator-activated receptor γ coactivator 1α (PGC-1α) messenger RNA (mRNA) protein, cytochrome C oxidase subunit IV (COX IV) protein abundance, citrate synthase activity, Nfe2l2, SOD2 (P < 0.05). Antioxidant reduced PGC-1α mRNA, COX enzyme activity 0.05) both rats.Vitamin suppresses biogenesis, regardless status.