Gld2-catalyzed 3′ monoadenylation of miRNAs in the hippocampus has no detectable effect on their stability or on animal behavior

• DOI: 10.1261/rna.056937.116
• Publication Date: 2016-08-06T02:18:17Z
• Authors: Fernanda Mansur; Maria Ivshina; Weifeng Gu; Laura Schaevitz; Emily Stackpole; Sharvari Gujja; Yvonne J.K. Edwards; Joel D. Richter
• Keywords: Knockout mouse; Wild type

Gld2, a noncanonical cytoplasmic poly(A) polymerase, interacts with the RNA binding protein CPEB1 to mediate polyadenylation-induced translation in dendrites of cultured hippocampal neurons. Depletion Gld2 from hippocampus leads deficit long-term potentiation evoked by theta burst stimulation. At least mouse liver and human primary fibroblasts, also 3′ monoadenylates thereby stabilizes specific miRNAs, which enhance mRNA translational silencing eventual destruction. These results suggest that would be likely monoadenylate stabilize miRNAs hippocampus, produce measurable changes animal behavior. We now report using knockout mice, there are detectable alterations miRNA monoadenylation when compared wild type, but these modifications no effect on stability. Moreover, we surprisingly find overt change behavior comparing wild-type mice. data indicate monoadenylation-mediated stability is cell type-specific has higher cognitive function.