A case for “StopGo”: Reprogramming translation to augment codon meaning of GGN by promoting unconventional termination (Stop) after addition of glycine and then allowing continued translation (Go)

• DOI: 10.1261/rna.487907
• Publication Date: 2007-04-25T02:09:14Z
• Authors: John F. Atkins; Norma M. Wills; Gary Loughran; Chih-Yu Wu; Krishna Parsawar; Martin D. Ryan; Chung-Hsiung Wang; Chad C. Nelson
• Keywords: Stop codon; Eukaryotic translation

When a eukaryotic mRNA sequence specifying an amino acid motif known as 2A is directly followed by proline codon, two nonoverlapping proteins are synthesized. From earlier work, the second protein to start with this codon and not created proteolysis. Here we identify C-terminal of upstream 2A-encoded product from Perina nuda picorna-like virus that glycine specified last 2A-encoding sequence. This example recoding where promotes unconventional termination after decoding continued translation beginning 3′ adjacent codon.