Detecting novel low-abundant transcripts in Drosophila

• DOI: 10.1261/rna.7239605
• Publication Date: 2005-05-27T18:47:32Z
• Authors: SANGGYU LEE; JINGYUE BAO; GUOLIN ZHOU; JOSHUA SHAPIRO; JINHUA XU; RUN ZHANG SHI; XUEMEI LU; TERRY CLARK; DEBORAH JOHNSON; YEONG C. KIM; CLAUDIA WING; CHARLES TSENG; MIN SUN; WEI LIN; JUN WANG; HUANMING YANG; JIAN WANG; WEI DU; CHUNG-I WU; XIUQING ZHANG; SAN MING WANG
• Keywords: Intergenic region

Increasing evidence suggests that low-abundant transcripts may play fundamental roles in biological processes. In an attempt to estimate the prevalence of eukaryotic genomes, we performed a transcriptome analysis Drosophila using SAGE technique. We collected 244,313 tags from expressed embryonic, larval, pupae, adult, and testicular tissue. From these tags, identified 40,823 unique tags. Our showed 55% are novel without matches currently known transcripts, most have low copy numbers. Further indicated represent loci outside annotated exons including intergenic intronic regions, antisense genome. study reveals presence significant number , highlights need isolate for further studies.