Impact of Heat Stress Exposure During Meiotic Maturation on Oocyte, Surrounding Cumulus Cell, and Embryo RNA Populations

• DOI: 10.1262/jrd.10-163m
• Publication Date: 2011-04-12T05:20:38Z
• Authors: Rebecca R. PAYTON; Louisa A. RISPOLI; Arnold M. SAXTON; J. Lannett EDWARDS
• Keywords: Germinal vesicle

To determine if reductions in developmental competence related to heat stress exposure were correlated with perturbations certain RNA populations, poly(A) RNA, total size distribution, and the abundance of transcripts (cyclin B1, GDF9, BMP15, polymerase, HSP70, 18S & 28S rRNA) examined oocytes matured at 38.5 or 41 C. Performing vitro fertilization resulted embryos for examining RNA. Relative germinal vesicle-stage oocytes, amount decreased similarly Total did not change during meiotic maturation up through 4 8-cell stage embryonic development. Blastocyst-stage had more RNA; those originating from heat-stressed than nonheat-stressed oocytes. Oocytes similar RIN values ratios rRNA, 18S/fast region, 18S/inter region. Values obtained blastocyst-stage cumulus cell which maturation. Culture C first 12 h no impact on distribution surrounding resultant embryos. Interestingly, however, blastocysts lower region compared other stages cells. Although biological significance these changes is unclear, differences molecular level emphasize importance minimizing maturation, intent obtain developmentally-competent