Carnosic acid improves porcine early embryonic development by inhibiting the accumulation of reactive oxygen species

• DOI: 10.1262/jrd.2020-086
• Publication Date: 2020-10-13T22:07:27Z
• Authors: Yan-xia PENG; Cheng-Zhen CHEN; Dan LUO; Wen-jie YU; Sheng-peng LI; Yue XIAO; Bao YUAN; Shuang LIANG; Xue-rui YAO; Nam-Hyung KIM; Hao JIANG; Jia-Bao ZHANG
• Keywords: Carnosic acid

Carnosic acid (CA), a natural catechol rosin diterpene, is used as an additive in animal feeds and human foods. However, the effects of CA on mammalian reproductive processes, especially early embryonic development, are unclear. In this study, we added to parthenogenetically activated porcine embryos vitro culture medium explore influence apoptosis, proliferation, blastocyst formation, reactive oxygen species (ROS) levels, glutathione (GSH) mitochondrial membrane potential, development-related gene expression. The results showed that supplementation with 10 μM during significantly improved cleavage rates, formation hatching total numbers cells compared no supplementation. More importantly, also GSH levels reduced ROS blastomeres, upregulated Nanog, Sox2, Gata4, Cox2, Itga5, Rictor expression, downregulated Birc5 Caspase3 These suggest can improve development by regulating oxidative stress. This study elucidates their potential mechanisms, provides new applications for improving quality vitro-developed embryos.