Effect of ovarian stimulation on developmental speed of preimplantation embryo in a mouse model

• DOI: 10.1262/jrd.2023-089
• Publication Date: 2024-03-17T22:18:24Z
• Authors: Mayuko KURUMIZAKA; Tatsuma YAO; Mikiko TOKORO; Noritaka FUKUNAGA; Yoshimasa ASADA; Kazuo YAMAGATA
• Keywords: Embryo quality

Ovarian stimulation protocols are widely used to collect oocytes in assisted reproductive technologies (ARTs). Although the influence of ovarian on embryo quality has been described, this issue remains controversial. Here, we analyzed developmental speed and chromosome segregation using live cell imaging. Female mice at proestrus stage were separated by appearance vagina as non-stimulation (–) group, other administered pregnant mare serum gonadotropin (PMSG) human chorionic (hCG) (+) groups. The cumulus-oocyte complexes from both groups inseminated with sperm suspensions same male mice. Fertilization rates capacities examined, frequency errors measured live-cell imaging a Histone H2B-mCherry probe. number fertilized obtained was 1.4-fold more frequent Stimulation group. rate stability did not differ between Image analysis showed that mean development group slightly higher than This increase seemed arise slight shortening 2- 4-cell stages third division lengths consequent synchronization cleavage timing each embryo, emergence an extremely rapidly developing subpopulation embryos. In conclusion, does necessarily affect but rather increases chances obtaining high-quality