Identification of Chemotaxis Sensory Proteins for Amino Acids in <i>Pseudomonas fluorescens</i> Pf0-1 and Their Involvement in Chemotaxis to Tomato Root Exudate and Root Colonization

• DOI: 10.1264/jsme2.me12005
• Publication Date: 2012-09-04T22:52:23Z
• Authors: Shota Oku; Ayaka Komatsu; Takahisa Tajima; Yutaka Nakashimada; Junichi Kato
• Keywords: Pseudomonas fluorescens; Exudate

Pseudomonas fluorescens Pf0-1 showed positive chemotactic responses toward 20 commonly-occurring l-amino acids. Genomic analysis revealed that P. possesses three genes (Pfl01_0124, Pfl01_0354, and Pfl01_4431) homologous to the aeruginosa PAO1 pctA gene, which has been identified as a chemotaxis sensory protein for amino When Pf01_4431, Pfl01_0124, Pfl01_0354 were introduced into pctB pctC triple mutant of PAO1, defective in acids, its transformants 18, 16, one acid, respectively. This result suggests are proteins acids their designated ctaA, ctaB, ctaC, The ctaA ctaB ctaC only weak Cys Pro but no other 18 indicating CtaA, CtaB, CtaC major Pf0-1. Tomato root colonization by strains was analyzed gnotobiotic competitive assay. It found less than wild-type strain, suggesting components exudate, an important role more cheA Pf0-1, is non-chemotactic, motile. chemoattractants also involved