Resveratrol inhibits TGF-β1–induced fibrotic effects in human pterygium fibroblasts

• DOI: 10.1265/ehpm.23-00020
• Publication Date: 2023-10-20T22:37:56Z
• Authors: Jianwu Fan; Shuang Wei; Xiaoyan Zhang; Li Chen; Xin Zhang; Yaping Jiang; Minjie Sheng; Yihui Chen
• Keywords: Myofibroblast; Viability assay

Resveratrol is a polyphenolic phytoalexin which has the properties of anti-oxidant, anti-inflammatory and anti-fibrotic effects. The aim this study was to investigate effects resveratrol in primary human pterygium fibroblasts (HPFs) elucidate underlying mechanisms.Profibrotic activation induced by transforming growth factor-beta1 (TGF-β1). expression profibrotic markers, including type 1 collagen (COL1), α-smooth muscle actin (α-SMA), fibronectin, were detected western blot quantitative real-time-PCR after treatment with various concentrations HPFs Relative signaling pathways downstream TGF-β1 Western assess mechanism. Cell viability apoptosis assessed using CCK-8 assay flow cytometry evaluate proliferation drug-induced cytotoxicity. migration contractile phenotype through wound healing gel contraction assay.The α-SMA, FN COL1 suppressed dose-dependent manner. Smad3, mitogen-activated protein kinase (p38 MAPK) phosphatidylinositol-3-kinase (PI3K) / B (AKT) activated TGF-β1, while attenuated those pathways. also inhibited cellular proliferation, phenotype, HPFs.Resveratrol inhibit TGF-β1-induced myofibroblast extra matrix synthesis HPFs, at least partly, regulating TGF-β/Smad3, p38 MAPK PI3K/AKT