Circular RNA Myosin Light Chain Kinase (MYLK) Promotes Prostate Cancer Progression through Modulating Mir-29a Expression

• DOI: 10.12659/msm.908009
• Publication Date: 2018-05-25T05:14:10Z
• Authors: Yuanqing Dai; Dongjie Li; Xiong Chen; Xinji Tan; Jie Gu; Mingquan Chen; Xiaobo Zhang
• Keywords: Male; 0301 basic medicine; Base Sequence; Down-Regulation; Prostatic Neoplasms; Apoptosis; RNA, Circular; Up-Regulation; 3. Good health; Gene Expression Regulation, Neoplastic; MicroRNAs; 03 medical and health sciences; Cell Movement; Cell Line, Tumor; Disease Progression; Humans; RNA; Neoplasm Invasiveness; Molecular Biology; Cell Proliferation

BACKGROUND:In developed countries, prostate cancer (PCa) is a frequently diagnosed with the second highest fatality rate. Circular RNAs (circRNAs) are class of endogenous non-coding (ncRNAs) stably expressed in cells and involved series carcinomas. However, few research studies have reported on role circRNAs PCa. MATERIAL AND METHODS:We used qRT-PCR to detect expression circMYLK (circRNA ID: hsa_circ_0141940) miR-29a PCa tissues cell lines. MTT, colony formation, TUNEL assays were performed analysis viability cells. Transwell wound scratch investigate invasion migration RESULTS:In present study, we confirmed that level was significantly higher samples than normal prostatic The upregulated circRNA-MYLK promoted proliferation, invasion, migration; however, si-circRNA-MYLK accelerated apoptosis. We also observed aforementioned function affected through targeting miR-29a. CONCLUSIONS:We an oncogene revealed novel mechanism underlying PC progression.