Qi-Xian Decoction Upregulated E-cadherin Expression in Human Lung Epithelial Cells and Ovalbumin-Challenged Mice by Inhibiting Reactive Oxygen Species-Mediated Extracellular-Signal-Regulated Kinase (ERK) Activation

• DOI: 10.12659/msm.922003
• Publication Date: 2021-02-22T17:24:22Z
• Authors: Lingling Tang; Linyun Zhu; Wei Zhang; Xiaoyan Yang; Qingge Chen; Ziyu Meng; Jinjin Liu; Yipeng Sun; Junsheng Hu; Zhenhua Ni; Xiongbiao Wang
• Keywords: 0301 basic medicine; Mice, Inbred BALB C; Ovalbumin; Animal Study; Epithelial Cells; Hydrogen Peroxide; Cadherins; Asthma; Up-Regulation; 3. Good health; Enzyme Activation; Disease Models, Animal; Mice; 03 medical and health sciences; Animals; Humans; Female; Phosphorylation; Extracellular Signal-Regulated MAP Kinases; Reactive Oxygen Species; Lung

BACKGROUND:Loss of the epithelial barrier is characterized by a reduction in E-cadherin expression and hallmark asthma. Qi-xian decoction (QXT) Chinese medicinal formula that has been used to effectively treat This study aimed investigate effect QXT on human lung 16HBE cells ovalbumin-challenged mice explore underlying molecular mechanism. MATERIAL AND METHODS:Ovalbumin (OVA)-induced were as model Real-time PCR Western blotting utilized examine mRNA protein levels. Lung tissue reactive oxygen species (ROS) levels evaluated using dichloro-dihydro-fluorescein diacetate (DCFH-DA). Serum superoxide dismutase (SOD) total antioxidant capacity (TAOC) measured via enzyme-linked immunosorbent assay (ELISA)-based analyses. or hydrogen peroxide (H₂O₂) vitro. RESULTS:We found treatment increased decreased extracellular-signal-regulated kinase (ERK) phosphorylation tissues OVA-challenged mice. also downregulated ROS serum SOD TAOC In vitro studies demonstrated generation induced H₂O₂ resulted cells, which was attenuated inhibition ERK signaling. Moreover, H₂O₂-induced downregulation expression, generation, activation restored with water ethanol extract. CONCLUSIONS:These data demonstrate one mechanism protects against asthma restore vivo inhibiting ROS-mediated activation.