Assessment of housekeeping genes as internal references in quantitative expression analysis during early development of oyster

• DOI: 10.1266/ggs.16-00007
• Publication Date: 2016-08-30T22:08:37Z
• Authors: Pin Huan; Hongxia Wang; Baozhong Liu
• Keywords: Reference Genes; Housekeeping gene; Glyceraldehyde 3-phosphate dehydrogenase

The early development of mollusks exhibits important characteristics from the developmental and evolutionary perspective. With increasing number genome-wide studies, accurate analyses quantitative gene expression during are impeded by lack validated reference genes. To improve situation, in this study, we analyzed stability seven candidate housekeeping genes Pacific oyster Crassostrea gigas: actin, glyceraldehyde-3-phosphate dehydrogenase (gapdh), α subunit elongation factor 1 (elf1α), adp-ribosylation (arf1), heterogeneous nuclear ribonucleoprotein q, ubiquitin-conjugating enzyme e2d2 ribosomal protein s18. We focused on 11 stages oocyte to D-veliger, which include crucial processes such as axis determination, gastrulation shell formation. Gene stabilities were assessed with three commonly used programs geNorm, NormFinder BestKeeper. Although results obtained varied some extent, general, arf1, elf1α gapdh highly ranked actin was poorly ranked. This analysis also indicated that multiple should be for normalization, concluded arf1-elf1α-gapdh internal references. findings study will help researchers obtain future bivalve mollusks.