The circadian clock mediates the response to oxidative stress in a cone photoreceptor‒like (661W) cell line via regulation of glutathione peroxidase activity

• DOI: 10.12688/f1000research.125133.2
• Publication Date: 2022-11-21T14:50:10Z
• Authors: Kenkichi Baba; Ting-Chung Suen; Varunika Goyal; Adam Stowie; Alec Davidson; Jason DeBruyne; Gianluca Tosini
• Keywords: 0301 basic medicine; Mice; Glutathione Peroxidase; Oxidative Stress; Aging; 03 medical and health sciences; Circadian Clocks; Retinal Cone Photoreceptor Cells; Animals; Research Article; Cell Line

<ns4:p><ns4:bold>Background</ns4:bold>: The mammalian retina contains an autonomous circadian clock that controls many physiological functions within this tissue. Our previous studies have indicated disruption of by removing <ns4:italic>Bmal1</ns4:italic> from the affects visual function, retinal circuitry, and cone photoreceptor viability during aging. In present study, we employed a mouse-derived photoreceptor‒like cell, 661W, to investigate which molecular mechanisms may modulate aging.</ns4:p><ns4:p> <ns4:bold>Methods</ns4:bold>: knockout (BKO) cells were generated 661W using CRISPR/Cas9 gene editing tool. Deletion was verified western blot monitoring <ns4:italic>Per2-luc</ns4:italic> bioluminescence rhythms. To effect removal on oxidative stress challenge, treated with hydrogen peroxide (H<ns4:sub>2</ns4:sub>O<ns4:sub>2</ns4:sub>,1 mM) for two hours then cell assessed. Cells also cultured harvested expression analysis antioxidant assay.</ns4:p><ns4:p> <ns4:bold>Results</ns4:bold>: data contain functional mediates response challenge <ns4:italic>in vitro</ns4:italic> such is no longer in BKO cell. We hypothesized due regulation intracellular defense mechanism. results revealed cells, mechanism showed time dependent variation , whereas there overall reduction mechanism, it variation.</ns4:p><ns4:p> <ns4:bold>Conclusions</ns4:bold>: work supported notion presence its ability underlying protect cones aging.</ns4:p>