A draft genome sequence for the Ixodes scapularis cell line, ISE6

• DOI: 10.12688/f1000research.13635.1
• Publication Date: 2018-03-08T15:50:18Z
• Authors: Jason R. Miller; Sergey Koren; Kari A. Dilley; Derek M. Harkins; Timothy B. Stockwell; Reed S. Shabman; Granger G. Sutton
• Keywords: Ixodes scapularis; Open peer review; Sequence (biology)

<ns4:p><ns4:bold>Background:</ns4:bold>The tick cell line ISE6, derived from<ns4:italic>Ixodes scapularis</ns4:italic>, is commonly used for amplification and detection of arboviruses in environmental or clinical samples.</ns4:p><ns4:p><ns4:bold>Methods:</ns4:bold>To assist with sequence-based assays, we sequenced the ISE6 genome single-molecule, long-read technology.</ns4:p><ns4:p><ns4:bold>Results:</ns4:bold>The draft assembly appears near complete based on gene content analysis, though it to lack some instances repeats this highly repetitive genome. The have separated haplotypes at many loci. DNA short read pairs, validation only, mapped a higher rate than they the<ns4:italic>Ixodes scapularis</ns4:italic>reference sequence.</ns4:p><ns4:p><ns4:bold>Conclusions:</ns4:bold>The could be useful filtering host sequence from data obtained cells infected pathogens.</ns4:p>