Secretomes of apoptotic mononuclear cells ameliorate neurological damage in rats with focal ischemia

• DOI: 10.12688/f1000research.4219.2
• Publication Date: 2014-10-28T11:51:54Z
• Authors: Patrick Altmann; Michael Mildner; Thomas Haider; Denise Traxler; Lucian Beer; Robin Ristl; Bahar Golabi; Christian Gabriel; Fritz Leutmezer; Hendrik Jan Anker...
• Keywords: 0303 health sciences; 03 medical and health sciences; Research Article; 3. Good health

<ns4:p>The pursuit of targeting multiple pathways in the ischemic cascade cerebral stroke is a promising treatment option. We examined regenerative potential conditioned medium derived from rat and human apoptotic mononuclear cells (MNC), rMNC<ns4:sup>apo sec</ns4:sup> hMNC<ns4:sup>apo sec</ns4:sup>, experimental stroke.</ns4:p><ns4:p>We performed middle artery occlusion on Wistar rats administered MNC-secretomes intraperitoneally two settings. Ischemic lesion volumes were determined 48 hours after ischemia. Neurological evaluations 6, 24 hours. Immunoblots conducted to analyze neuroprotective signal-transduction primary glia neurons. Neuronal sprouting assays neurotrophic factors both plasma quantified using ELISA.</ns4:p><ns4:p>Administration as well significantly reduced by 36% 37%, respectively. examinations revealed improvement groups. Co-incubation astrocytes, Schwann neurons with resulted activation several signaling cascades associated regulation cytoprotective gene products enhanced neuronal <ns4:italic>in vitro</ns4:italic>. Analysis high levels brain factor (BDNF).</ns4:p><ns4:p>Our data indicate that elicit effects have undergone stroke.</ns4:p>