Cell Cycle Checkpoint and Apoptosis Induction in Glioblastoma Cells and Fibroblasts Irradiated with Carbon Beam

• DOI: 10.1269/jrr.06081
• Publication Date: 2007-07-19T05:49:37Z
• Authors: Koji TSUBOI; Takashi MORITAKE; Yukihiro TSUCHIDA; Koichi TOKUUYE; Akira MATSUMURA; Koichi ANDO
• Keywords: Radioresistance; Radiosensitivity

This study was conducted in order to evaluate the cytotoxicity of high linear-energy-transfer (LET) ionizing radiation (IR) on glioblastoma cells and fibroblasts using different modes cell inactivation assays. Two human lines with or without p53-mutation, were used as materials. Gamma rays 290 MeV/u carbon beams LET values 20, 40, 80 keV/μm used. To inactivation, we colony formation assay, morphological detection apoptosis, flow-cytometry. Serial expressions p53 p21 analyzed by immunoblotting. High-LET IR reduced reproductive potency these identical levels spite differences gamma-sensitivity, yield death correlated values. A p53-wild-type line demonstrated a higher apoptosis than other lines, whereas hardly displayed any indicating senescence-like growth arrest even after IR. p53-mutant tumor very low prominent G2/M arrest. Results radiosensitivity differ according what mode is selected. While depend G1 block IR, blocks may play crucial roles radioresistance cells.