Live Imaging of Radiation-Induced Apoptosis by Yolk Injection of Acridine Orange in the Developing Optic Tectum of Medaka

• DOI: 10.1269/jrr.09043
• Publication Date: 2009-12-04T05:54:29Z
• Authors: Takako YASUDA; Shoji ODA; Yuji ISHIKAWA; Tomomi WATANABE-A...; Masayuki HIDAKA; Hiroshi YASUDA; Kazunori ANZAI; Hiroshi MITANI
• Keywords: Acridine orange; Yolk

To observe the sequential radiation-induced apoptosis in a living embryo, we injected Acridine Orange (AO) solution into yolk of embryo and visualized developing optic tectum (OT). Medaka embryos at stage 28, when neural cells proliferate rapidly OT, were irradiated with 5 Gy X-rays which is non-lethal dose for hatching. The hatched normally without morphological abnormalities their brains, even though large number apoptotic induced transiently OT. By injection, OT distinguished as AO-positive small nuclei 3 h after irradiation. At 8-10 irradiation, rosette-shaped clusters obviously marginal tectal regions where are proliferating intensely. became bigger more obvious, but did not increase up to 24 irradiation completely disappeared 49 This characteristic appearance nuclei/clusters good agreement our previous results, based on examination fixed specimens stained AO by injection peri-vitelline space, suggesting that AO-yolk method highly reliable detecting embryos. live imaging expected reveal details dynamics responses brain other tissues.