Mapping of a major QTL associated with protein content on chromosome 2B in hard red winter wheat (<i>Triticum aestivum</i> L.)

• DOI: 10.1270/jsbbs.16026
• Publication Date: 2016-07-04T22:03:25Z
• Authors: Yohei Terasawa; Miwako Ito; Tadashi Tabiki; Koichi Nagasawa; Koichi Hatta; Zenta Nishio
• Keywords: 0301 basic medicine; 2. Zero hunger; 03 medical and health sciences; Research Paper

A quantitative trait locus (QTL) controlling wheat grain protein content (GPC) and flour protein content (FPC) was identified using doubled haploid (DH) lines developed from a cross between the hard red winter wheat variety 'Yumechikara' with a high protein content used for bread making, and the soft red winter wheat 'Kitahonami' with a low protein content used for Japanese white salted noodles. A single major QTL, QGpc.2B-yume, was identified on the short arm of wheat chromosome 2B for both the GPC and FPC over 3 years of testing. QGpc.2B-yume was mapped on the flanking region of microsatellite marker Xgpw4382. The DH lines grouped by the haplotype of the closest flanking microsatellite marker Xgpw4382 showed differences of 1.0% and 1.1% in mean GPC and FPC, respectively. Yield-component-related traits were not affected by the haplotype of QGpc.2B-yume, and major North American hard red winter wheat varieties showed the high-protein haplotype. Unlike Gpc-B1 derived from tetraploid wheat, QGpc.2B-yume has no negative effects on yield-component-related traits and should be useful for wheat breeding to increase GPC and FPC.