Genetic analysis of the low-amylose characteristics of rice cultivars Oborozuki and Hokkai-PL9

• DOI: 10.1270/jsbbs.60.187
• Publication Date: 2010-09-08T06:10:23Z
• Authors: Ikuo Ando; Hiroyuki Sato; Noriaki Aoki; Yasuhiro Suzuki; Hideyuki Hirabayashi; Makoto Kuroki; Hiroyuki Shimizu; Tsuyu Ando; Yoshinobu Takeuchi
• Keywords: Marker-Assisted Selection

Control of the amylose content is a major strategy for breeding rice with improved eating quality. To identify low-amylose gene cultivar Oborozuki, quantitative trait loci (QTL) and sequence analyses were conducted on 90 progeny obtained by crossing two japonica cultivars Hokka287 (a donor parent Oborozuki) Hokkai-PL9. One QTL low was detected WX1 locus short arm chromosome 6. This designated Wx1-1 Hokkai287 allele, novel allelic 37-bp deletion in intron 10. Homozygote genotype decreased 7.8%. Primer sets Wx-U1L3 designed to amplify genome region containing clearly differentiated from others. DNA marker useful contents. Another QTL, qAC9.3, 9. Hokkai-PL9-homozygote allele qAC9.3 2.6%. showed that Hokkai-PL9 have an additive effect decreasing content. The genes are tools marker-assisted selection when controlled