Characterization and fine mapping of thermo-sensitive chlorophyll deficit mutant1 in rice (<i>Oryza sativa</i> L.)

• DOI: 10.1270/jsbbs.65.161
• Publication Date: 2015-04-27T22:02:00Z
• Authors: Jin Liu; Jiayu Wang; Xiaoyun Yao; Yu Zhang; Jinquan Li; Xiaoxue Wang; Zhengjin Xu; Wenfu Chen
• Keywords: Tiller (botany); Wild type; Chlorophyll b

Chlorophyll content is one of the most important traits controlling crop biomass and economic yield in rice. Here, we isolated a spontaneous rice mutant named thermo-sensitive chlorophyll deficit 1 (tscd1) derived from backcross recombinant inbred line population. tscd1 plants grown normally seedling to tiller stages showed yellow leaves with reduced content, but no significant differences after booting stage. At temperatures below 22°C, obvious yellowish phenotype. With increasing temperature, gradually turned green approached normal wild type color. Wild had obviously different chloroplast structures photosynthetic pigment precursor contents, which resulted underdevelopment chloroplasts phenotype tscd1. Genetic analysis indicated that character was controlled by recessive nuclear gene. Through map-based cloning, located gene 34.95 kb region on long arm chromosome 2, containing two BAC clones eight predicted candidate genes. Further characterization underway. Because it has before stage little influence growth vigor, may play role ensuring purity hybrids.