Screening, Characterization, and Cloning of the Gene forN-Carbamyl-D-Amino acid Amidohydrolase from Thermotolerant Soil Bacteria

• DOI: 10.1271/bbb.62.882
• Publication Date: 2005-03-29T06:05:27Z
• Authors: Yasuhiro IKENAKA; Hirokazu NANBA; Yukio YAMADA; Kazuyoshi YAJIMA; Masayuki TAKANO; Satomi TAKAHASHI
• Keywords: Cloning (programming); Amidohydrolase; Characterization

For the production of D-amino acids, thermotolerant bacteria producing N-carbamyl-D-amino acid amidohydrolase were isolated from soil by enrichment culture at 45 degrees C with acids as sole nitrogen source. The enzyme activities and substrate specificities these strains examined resting cells reaction. One enzymes, produced Pseudomonas sp. strain KNK003A, was purified characterized, amino its N-terminal region sequenced. A DNA fragment containing gene for a thermostable then cloned into Escherichia coli. encoded peptide 312 calculated molecular weight 35,000. similarity deduced sequences this related mesophile, Agrobacterium KNK712, 60%. database searched similar sequences.