Isolation and characterization of multiple forms of rat liver lysozymes.

• DOI: 10.1271/bbb1961.45.1817
• Publication Date: 2011-07-13T05:54:06Z
• Authors: V. SIDHAN; S. GURNANI
• Keywords: Sephadex; Polyacrylamide

Multiple forms of lysozyme found in the rat liver were isolated and characterized from cellular organelles. Isolation enzymes was achieved by Sephadex gel filtration chromatography on CM cellulose-column. The purity preparations examined electrophoresis polyacrylamide gel. nuclear moved as a single band indicating homogeneity, whereas other subcellular lysozymes appeared heterogeneous due to presence more than one band, thus showing partial purity. Although similar with respect enzymatic properties, pH, buffer molarity optima electrophoretic mobility, differences observed elution patterns, responses inhibitor heat sensitivity. Nuclear distinctly different these criteria compared lysozymes.