Comparison of AndroMed and Triladyl Extender for Freezing of Jeju Native Black Bull Semen

• DOI: 10.12749/rdb.2013.37.3.155
• Publication Date: 2013-11-18T12:32:33Z
• Authors: Sang-Rae Cho; In-Chel Cho; Sang-Hyun Han; Tae-Young Kang; Won-Mo Cho; Hyun-Seok Chae; Nam-Young Kim; Yong-Sang Park; Yong-Jun Kang; Young-Hoon Kim; Yeoung-Gyu Ko; Hyun-Jong Kim; Moon-Suck Ko
• Keywords: 0402 animal and dairy science; 04 agricultural and veterinary sciences

This study was carried out to investigate synthetic extender for semen cryopreservation of Jeju Native Black Bull. The collected using an artificial vagina and transported the laboratory. diluted 1:1 by Tris-Egg yolk contrifuged in 1,500 rpm 15 minutes. supernatant removed. pellect final sperm concentration 2×108/ml doubling every 30 minutes at 4℃ cold chamber. equilibrated 4 hours chamber packed 0.5 ml straw. straws were located above 5 cm 10 height duration affect freezing speed temperature. frozen straw plunged LN2. presented examined viability motility after thawed 37℃ water bath. Frozen-thawed evaluated viability, membrane integrity acrosome integrity. Post-thawed has been significantly higher (p<0.05) fresh (93.27±1.62%) than frozen-thawed (73.34±3.27%). However, there no significant differences between dead cell rate (7.35±2.63 vs, 13.71±2.85). In motility, Triladyl AndroMed Extender, different (72.86±2.83 81.47±2.48), similarly, rates similar (18.41±3.42% 17.26±4.25). results our suggest that showed more positive effect on spermatozoa bull semen.