Selection of a monoclonal antibody by ELISA, immunoblotting and Quartz Crystal Microbalance technology for immunohistochemical detection of Mycoplasma mycoides subsp. mycoides.

• DOI: 10.12834/vetit.435.2084.2
• Publication Date: 2016-07-15
• Authors: Mirella Luciani; Gisella Armillotta; Laura Manna; Antonella Ciarelli; Tiziana Di Febo; Ivanka Krasteva; Attilio Pini; Flavio Sacchini; Anna Rita D’Angelo
• Keywords: Contagious bovine pleuropneumonia; Mycoplasma mycoides; clone (Java method); Pleuropneumonia

An immunohistochemical (IHC) technique was optimised using a monoclonal antibody (MAb) to detect Mycoplasma mycoides subsp. (Mmm), the agent of Contagious Bovine Pleuropneumonia (CBPP), in sections lung tissue. A panel MAbs produced and screened for Mmm speci city cross-reactivity against other mycoplasmas belonging not cluster, parallel indirect ELISA (i-ELISA) Immunoblotting (IB). Based on i-ELISA IB characterization data, 1 MAb (clone 3G10E7) selected its highest nity vs con rmed by Quartz Crystal Microbalance (QCM) technology. Afterwards, IHC analyses were conducted compare 3G10E7 rabbit c hyperimmune serum tissue CBPP infected negative animals. Results suggest that screening ELISA, IB, QCM technology enables select high target MAbs. Immunohistochemical results demonstrated improved performances, showing reduced background staining no bovis, which is responsible pneumonia cattle.