Quantitative and molecular analyses of genetic risk: a study with ionizing radiation.

• DOI: 10.1289/ehp.93101s3213
• Publication Date: 2011-06-10T15:10:47Z
• Authors: A W Hsie; Z Xu; Y Yu; J An; M L Meltz; J L Schwartz; P Hrelia
• Keywords: Phosphoribosyltransferase; Ethyl methanesulfonate; Hypoxanthine Phosphoribosyltransferase; Adenine phosphoribosyltransferase

Mammalian cells in culture have been used to study the genetic effects of physical and chemical agents. We Chinese hamster ovary (CHO) cells, clone K1-BH4, quantify mutations at X-linked, large (35 kb) hypoxanthine-guanine phosphoribosyltransferase (hprt) locus (the CHO/HPRT assay) induced by environmental By transfecting an hprt-deletion mutant CHO cell line with plasmid vector pSV2gpt, we isolated a transformant, AS52. AS52 carry single functional copy autosomal, small (456 bp) xanthine-guanine (gpt) gene bacterial equivalent mammalian hprt gene; AS52/GPT assay). found that ionizing radiations such as X-rays neutrons oxidative genotoxic chemicals Adriamycin, bleomycin, hydrogen peroxide, potassium superoxide are much more mutagenic gpt than K1-BH4 cells. The hypermutability probably results from higher recovery multilocus deletion mutants rather yield mutants. These demonstrate use alone could lead underestimate risk these Analyses mutation spectrum using polymerase chain reaction-based screening DNA sequencing procedure showed high proportion HPRT- GPT- mutations. Thus, both frequency need be considered assessing radiation chemicals.