Prevalence of Extended-Spectrum β-Lactamase-Producing Escherichia coli and Klebsiella pneumoniae in Food-Producing Animals

• DOI: 10.1292/jvms.11-0372
• Publication Date: 2011-10-07T05:39:18Z
• Authors: Midori HIROI; Fumie YAMAZAKI; Tetsuya HARADA; Naomi TAKAHASHI; Natsuko IIDA; Yoshihiro NODA; Miya YAGI; Tomohiro NISHIO; Takashi KANDA; Fumihiko KAWAMORI; Kanji SUGIYAMA; Takashi MASUDA; Yukiko HARA-KUDO; Norio OHASHI
• Keywords: DNA, Bacterial; 0301 basic medicine; 2. Zero hunger; Swine; Microbial Sensitivity Tests; Polymerase Chain Reaction; beta-Lactamases; Electrophoresis, Gel, Pulsed-Field; 3. Good health; Feces; Klebsiella pneumoniae; 03 medical and health sciences; Escherichia coli; Food Microbiology; Animals; Cattle; Chickens; Plasmids

To evaluate the diversity of extended-spectrum β-lactamases (ESBL) genes among food-producing animals, 48 isolates ESBL-producing Escherichia coli were obtained from rectal samples broilers, layers, beef cattle and pigs, at slaughterhouse level. ESBL-carrying E. isolated 60.0% individual broiler samples, 5.9% 12.5% 3% pigs. One Klebsiella pneumoniae was a broiler. The ESBL-positive broilers harbored various ESBL genes: bla (SHV-12), bla(CTX-M-2), bla(CTX-M-14), bla(CTX-M-15) bla(CTX-M-44). plasmid DNAs analyzed by restriction patterns. Homogeneous band patterns yielded in those K. harboring bla(CTX-M-2) gene different farms. No genetic relation between 2 CTX-M-14 strains found pulsed-field gel electrophoresis, although plasmids these strains, farms, similar to each other. This study provides evidence that proliferation CTX-M-producing is due growth indigenous possible emergence acquired CTX-M horizontal transfer coliforms should be controlled critical importance cephalosporins zoonotic potential bacteria.