Proliferation Capacity, Neuronal Differentiation Potency and Microstructures after the Differentiation of Canine Bone Marrow Stromal Cells into Neurons

• DOI: 10.1292/jvms.11-0388
• Publication Date: 2012-02-15T05:36:27Z
• Authors: Kazuya EDAMURA; Koji KURIYAMA; Kenichi KATO; Rei NAKANO; Kenji TESHIMA; Kazushi ASANO; Tsuneo SATO; Shigeo TANAKA
• Keywords: Neurons; 0303 health sciences; 03 medical and health sciences; Dogs; Microscopy, Electron, Scanning; Animals; Cell Differentiation; Mesenchymal Stem Cells; Cell Proliferation

We examined the proliferation capacity and neuronal differentiation potency of canine bone marrow stromal cells (BMSCs). In addition, the microstructures of neuron-like cells after neuronal differentiation were observed under a scanning electron microscope. Canine BMSCs grew to confluency at 10.0 ± 2.5 days, and 3.8 ± 2.1 × 10(6) BMSCs were collected in one passage. Approximately 65% of canine BMSCs changed to neuron-like morphology after neuronal differentiation, and nearly all neuron-like cells stained positive against neuron-specific enolase. In addition, microstructures such as the cellular organelles, filaments and growth cones of these cells bore a close resemblance to those of the original mature neurons. These results suggested that canine BMSCs might be capable of differentiating into neurons.