Prevalence and Characteristics of <i>Salmonella</i> spp. Isolated from Poultry Slaughterhouses in Korea

• DOI: 10.1292/jvms.13-0093
• Publication Date: 2013-05-12T22:41:16Z
• Authors: Dong Hwa BAE; Hirut Kidie DESSIE; Ho Jeong BAEK; Seong Guk KIM; Hee Soo LEE; Young Ju LEE
• Keywords: 0301 basic medicine; Salmonella Infections, Animal; Genotype; Geography; Poultry; Electrophoresis, Gel, Pulsed-Field; 3. Good health; 03 medical and health sciences; Species Specificity; Salmonella; Drug Resistance, Bacterial; Republic of Korea; Prevalence; Animals; Bacteriophage Typing; Abattoirs; Poultry Diseases

Poultry products have consistently been identified as important sources of Salmonella infection in humans, because vertical transfer of infection from breeding hens to progeny is an important aspect of the epidemiology of Salmonella spp. infection within the poultry industry. The aim of this study was to estimate the prevalence of Salmonella contamination in poultry products from 15 different located geographical areas from among the 50 poultry slaughterhouses authorized to operate in Korea and to characterize all the isolates by genotyping, phage typing and antibiotic resistance pattern. Salmonella was isolated from 10 (66.7%) of the first and 5 (33.3%) of the last chilling waters and from 32 (42.7%) carcasses originating from 9 slaughterhouses. The major prevalent serotypes of Salmonella originating from 2 duck slaughterhouses and 13 chicken slaughterhouses tested were S. Typhimurium and S. Enteritidis, respectively. Regarding the characteristics of their antibiotic resistance, 8 of the 11 ampicillin resistant (AmR) isolates carried blaTEM only, two carried blaTEM and blaCTX-M-14 and one carried blaCTX-M-3 and only one AmR isolate with the blaCTX-M-3 β-lactamase gene was an ESBL-producing Salmonella strain. Twenty-seven Salmonella isolates showed nalidixic acid resistance with a mutation at amino acid codon Asp87 in gyrA and no mutation in the parC gene. In all the phenotypic and genotypic properties of the 18 S. Enteritidis and 8 S. Typhimurium based on PFGE, phage types and antibiotic resistance pattern, the predominant patterns were XEI/BEI-PT32a-NaR (n = 5) and XTI/BTI-RNDC-no resistant antibiotics (n = 6), respectively.