Effects of a Bacteria-Based Probiotic on Subpopulations of Peripheral Leukocytes and Their Cytokine mRNA Expression in Calves

• DOI: 10.1292/jvms.13-0370
• Publication Date: 2013-10-16T23:04:05Z
• Authors: Abdul Qadir QADIS; Satoru GOYA; Minoru YATSU; Yu-uki YOSHIDA; Toshihiro ICHIJO; Shigeru SATO
• Keywords: 2. Zero hunger; 0301 basic medicine; Analysis of Variance; Time Factors; Interleukin-6; Tumor Necrosis Factor-alpha; Probiotics; Enterococcus faecium; 04 agricultural and veterinary sciences; Real-Time Polymerase Chain Reaction; 3. Good health; 0403 veterinary science; Interferon-gamma; 03 medical and health sciences; T-Lymphocyte Subsets; Internal Medicine; Clostridium butyricum; Leukocytes, Mononuclear; Animals; Cytokines; Cattle; RNA, Messenger; 14. Life underwater; Lactobacillus plantarum

Eight Holstein calves (10 ± 3 weeks) were used to examine the interaction between a bacteria-based probiotic agent (probiotic) and the function of peripheral blood mononuclear cells (PBMCs). The probiotic, consisting of Lactobacillus plantarum, Enterococcus faecium and Clostridium butyricum, was administered orally at 3.0 g/100 kg body weight to calves once daily for 5 consecutive days. Calves given the vehicle alone with no probiotic served as the control. In the treatment group, increases in numbers of CD282(+) (TLR2) monocytes, CD3(+) T cells and CD4(+), CD8(+) and WC1(+) γδ T cell subsets were noted on day 7 post-placement compared to predose day and the control group. Expression of interleukin (IL)-6, interferon-gamma (INF-γ) and tumor necrosis factor-alpha (TNF-α) was elevated in peripheral leukocytes on days 7 and 14. These results suggest that peripheral blood leukocytes in healthy calves may be stimulated via the gastrointestinal microbiota, which was increased by the oral probiotic treatment, with overall stability of the rumen bacterial flora. The 5-day repeated administration of a bacteria-based probiotic may enhance cellular immune function in weaned calves.