Immune-Stimulatory Effects of a Bacteria-Based Probiotic on Peripheral Leukocyte Subpopulations and Cytokine mRNA Expression Levels in Scouring Holstein Calves

Specific-pathogen-free TLR5 Clostridium butyricum
DOI: 10.1292/jvms.13-0534 Publication Date: 2014-01-22T23:27:32Z
ABSTRACT
Subpopulations of peripheral leukocytes and cytokine mRNA expression levels were evaluated in scouring healthy Holstein calves (age 10 ± 5 days; n=42) treated with a probiotic consisting Lactobacillus plantarum, Enterococcus faecium Clostridium butyricum. The assigned to the or group then subdivided into pathogen-positive (n=8), control pathogen-negative (n=6), (n=6) (n=8) groups. A single dose (3.0 g/100 kg body weight) was given each calf treatment groups for days. Blood samples collected on first day scour occurrence (day 0) 7. In calves, smaller leukocyte subpopulations noted 0. numbers CD3(+) T cells; CD4(+), CD8(+) WC1(+) γδ cell subsets; CD14(+), CD21(+) CD282(+) (TLR2) cells significantly increased Furthermore, interleukin-6, tumor necrosis factor-alpha interferon-gamma elevated recovered number lower level might be induced by calves. Repeated administration stimulate cellular immunity encourage recovery from pre-weaning
SUPPLEMENTAL MATERIAL
Coming soon ....
REFERENCES (39)
CITATIONS (15)