Bone Regeneration by the Combined Use of Tetrapod-Shaped Calcium Phosphate Granules with Basic Fibroblast Growth Factor-Binding Ion Complex Gel in Canine Segmental Radial Defects

• DOI: 10.1292/jvms.14-0027
• Publication Date: 2014-03-25T23:09:26Z
• Authors: Muneki HONNAMI; Sungjin CHOI; I-li LIU; Wataru KAMIMURA; Tetsushi TAGUCHI; Hironori HOJO; Nobuyuki SHIMOHATA; Shinsuke OHBA; Hiroyuki KOYAMA; Ryohei NISHIMURA; Ung-il CHUNG; Nobuo SASAKI; Manabu MOCHIZUKI
• Keywords: Apposition; Bone Growth

The effect of tetrapod-shaped alpha tricalcium phosphate granules (Tetrabones® [TB]) in combination with basic fibroblast growth factor (bFGF)-binding ion complex gel (f-IC gel) on bone defect repair was examined. Bilateral segmental defects 20-mm long were created the radius 5 dogs, stabilized a plate and screws implanted 1 following: TB (TB group), bFGF solution (TB/f f-IC (TB/f-IC group). Dogs euthanized 4 weeks after surgery. Radiographs showed well-placed equal osseous callus formation all groups. Histomorphometry revealed that number vessels volume new TB/f-IC group significantly higher than those other However, no significant differences neovascularization observed between TB/f Furthermore, difference lamellar or rate mineral apposition among These results suggest increased might have been because promotion by gel. Therefore, combinatorial method may provide suitable scaffold for regeneration large defects.