Molecular detection of zoonotic tick-borne pathogens from ticks collected from ruminants in four South African provinces

• DOI: 10.1292/jvms.15-0170
• Publication Date: 2015-07-30T22:20:39Z
• Authors: Khethiwe MTSHALI; ZTH KHUMALO; Ryo NAKAO; Dennis J. GRAB; Chihiro SUGIMOTO; OMM THEKISOE
• Keywords: Amblyomma variegatum; Coxiella burnetii; Anaplasma phagocytophilum; Amblyomma; Rhipicephalus; Tick-borne disease

Ticks carry and transmit a remarkable array of pathogens including bacteria, protozoa viruses, which may be veterinary and/or medical significance. With little to no information regarding the presence tick-borne zoonotic or their known vectors in southern Africa, aim our study was screen for Anaplasma phagocytophilum, Borrelia burgdorferi, Coxiella burnetii, Rickettsia species Ehrlichia ruminantium ticks collected identified from ruminants Eastern Cape, Free State, KwaZulu-Natal Mpumalanga Provinces South Africa. The most abundant tick this were Rhipicephalus evertsi (40%), (35%), Amblyomma hebraeum (10%) decoloratus (14%). A total 1634 collected. DNA extracted, samples subjected PCR amplification sequencing. overall infection rates with target four as follows: A. 7%; C. E. ruminantium, 28%; spp., 27%. B. burgdorferi could not confirmed. findings show that are present studied African provinces. This will aid epidemiology diseases country well raising awareness about such veterinary, tourism sectors, they affected.