Composition of fecal microbiota of laboratory mice derived from Japanese commercial breeders using 16S rRNA gene clone libraries

• DOI: 10.1292/jvms.15-0454
• Publication Date: 2016-02-19T23:31:03Z
• Authors: Ryoko NOZU; Masami UENO; Nobuhito HAYASHIMOTO
• Keywords: clone (Java method); Library; Clostridiales

The fecal microbiota of six mice derived from three Japanese commercial breeders was analyzed by using 16S rRNA gene clone libraries to construct a database for analyzing the gut laboratory mice. 566 clones were obtained generated DNA samples BALB/c, C57BL/6N, DBA/2 and ICR Among these clones, there 446 unique sequences. When grouped at 98% similarity level, sequences consisted 103 Clostridiales, 43 Bacteroidales, 5 Lactobacillus 3 Erysipelotricaceae, as well 11 other phyla.