Genomic characterization and distribution of bovine foamy virus in Japan

• DOI: 10.1292/jvms.20-0429
• Publication Date: 2020-09-13T22:05:43Z
• Authors: Mari OKAMOTO; Keisuke OGUMA; Nanako YAMASHITA-...; Toshihiro ICHIJO; Shinichi HATAMA; Maiko ENDO; Maya ISHIKAWA; Takeshi HAGA
• Keywords: 0301 basic medicine; 0303 health sciences; Cattle Diseases; Genomics; Enzootic Bovine Leukosis; 3. Good health; 03 medical and health sciences; Japan; Virology; Leukemia Virus, Bovine; Animals; Spumavirus; Cattle; Female; Phylogeny

Bovine foamy virus (BFV) is distributed through worldwide cattle herds. Although the biological features of BFV are not well understood, appearance of clinical manifestation by superinfection with other microorganisms is inferred. In Japan, reports of genomic characterizations and epidemiology of this virus are limited. In this study, we performed whole genomic sequencing of BFV strains Ibaraki and No.43, which were isolated in this country. Additionally, we investigated BFV in geographically distant four daily farms in Japan, to estimate the distribution of BFV and its correlation to bovine leukemia virus (BLV). BFV was distributed throughout Japan; the average positive rate was 12.7%. The nucleotide sequence identities of the isolates were 99.6% when compared with BFV strain isolated in the USA. The phylogenetic tree using env gene sequence showed strains Ibaraki, No.43 and Kagoshima were sorted in the same cluster including the USA and Chinese strains, while Hokkaido strain was in the other cluster including European strains. Although no clear correlation between BFV and BLV could be found, BFV and BLV infections were likely to increase with ages. Our data on epidemiology and characteristics of BFV will provide important information to reveal biological features of BFV.