Possible correlation of specific bacteria detected by using a novel screening method and periodontal disease in dogs

• DOI: 10.1292/jvms.25-0004
• Publication Date: 2025-04-13T22:06:47Z
• Authors: Kaori TAKAHASHI; Eriko ARIMA; Hayate SAWAYAMA; Atsufumi SUDA; Naoki MIURA; Yoshinori SHIRAKATA; Satoshi ITO; Yasuo SUDA

This study sought to create a simple method for screening and detecting oral bacteria associated with periodontal disease as well identify the bacterial species that had strongest correlation severity in dogs. Fifty-two dogs from Kagoshima University Veterinary Teaching Hospital were included. The status, which included gingival recession, root surface exposure, redness, was determined through visual examination. Oral swab samples taken each dog's margin or tooth cervix using sterile cotton following standardized protocol. collected applied our nanotechnology named sugar chain immobilized magnetized gold nanoparticle/magnetic microparticle (SGNP/MMP) obtain DNA, then subjected qualitative semiquantitative PCR analyses 6 genera 8 of bacteria: Porphyromonas gingivalis (P. gingivalis), Tannerella forsythia (T. forsythia), Treponema denticola denticola), Actinobacillus actinomycetemcomitans (A. actinomycetemcomitans), Fusobacterium nucleatum (F. nucleatum), Prevotella intermedia intermedia), gulae gulae), cangingivalis cangingivalis). most strongly correlated T. (associated recession), followed by slight total P. gingivalis+P. gulae, but no significant found gulae. Within scope this study, analytical used may represent useful standard specific bacteria, quantity is linked