Biochemical and Histological Study of Rat Liver and Kidney Injury Induced by Cisplatin

• DOI: 10.1293/tox.26.293
• Publication Date: 2013-10-14T23:03:09Z
• Authors: Sarawoot PALIPOCH; Chuchard PUNSAWAD
• Keywords: Malondialdehyde; Blood urea nitrogen; Nephrotoxicity

Cisplatin is a chemotherapeutic agent widely used in treatment of several cancers. It documented as major cause clinical nephrotoxicity and hepatotoxicity. The purpose this study was to investigate the involvement oxidative stress pathogenesis cisplatin-induced liver kidney injury. Wistar rats were divided into four groups. Group 1 (control) intraperitoneally (IP) injected with single dose 0.85% normal saline. Groups 2, 3 4 IP doses cisplatin at 10, 25 50 mg/kg body weight (BW), respectively. At 24, 48, 72, 96 120 h after injection, BW, levels alanine aminotransferase (ALT), aspartate (AST), blood urea nitrogen (BUN), creatinine, malondialdehyde (MDA), activity superoxide dismutase (SOD) histology evaluated. caused reduction BW groups all post injection intervals. serum ALT, AST, BUN creatinine MDA markedly increased especially 48 72 h, whereas SOD decreased injection. Liver sections revealed moderate severe congestion dilation hepatic artery, portal vein bile duct disorganization cords cisplatin. Kidney illustrated mild tubular necrosis Therefore, implicated injury causing biochemical histological alterations.