Zingiber cassumunar ROXb. and its active constituent inhibit MMP-9 direct activation by house dust mite allergens and MMP-9 expression in PMA-stimulated human airway epithelial cells

• DOI: 10.12932/ap0490.33.1.2015
• Publication Date: 2014-10-23T02:37:06Z
• Authors: Orapan Poachanukoon; Ladda Meesuk; Napaporn Pattanac...; Paopanga Monthana...; Thaweephol Dechat...; Sittichai Koonton...
• Keywords: Phorbol; Zymography; Cleavage (geology)

House dust mite (HDM) induced matrix metalloproteinase (MMP)-9 plays a role in asthma. Zingiber cassumunar Roxb. (Phlai Thai) has been used folk medicine for asthma treatment.We investigated effects of Phlai and its constituent (E)-4-(3',4'-dimethoxyphenyl)but-3-en-1-ol (compound D) on the cleavage pro- MMP-9 by HDM. The these compounds phorbol 12-myristate 13-acetate (PMA)- gene protein expression airway epithelial cells (NCI-H292) were also investigated.Pro-MMP-9 was directly activated vitro with HDM presence or absence ethanolic extracts compound D 1 hour. amount determined using gelatin zymography. To study cellular response Phlai, NCI-H292 pretreated crude 2 hours, then stimulated PMA 48 hours. mRNA RT-PCR Western blotting, respectively. activity zymography.Crude (0.25 - 2.0 mg/ml) (0.5 4.0 inhibited Furthermore, (100 D, at concentrations 50 100 mg/ml, attenuated PMA-induced cells. These suppressed release from cells.The extract Z. active pro-MMP-9 They synthesis human