Comparison of cryopreserved amniotic membrane and umbilical cord tissue with dehydrated amniotic membrane/chorion tissue

• DOI: 10.12968/jowc.2014.23.10.465
• Publication Date: 2014-10-08T17:21:26Z
• Authors: M. Cooke; E.K. Tan; C. Mandrycky; H. He; J. O'Connell; S.C.G. Tseng
• Keywords: Amnion

Objective: To evaluate how the different processing methods cryopreservation and dehydration affect structural integrity biological composition of key signalling molecules within amniotic membrane umbilical cord tissues. Method: We directly compared cryopreserved (AM) (UC) tissues with dehydrated membrane/chorion (dHACM) tissue using biochemical functional assays including histological histochemical staining, BCA, agarose gel electrophoresis, western blot, ELISA, proliferation cell death assays. Results: Cryopreservation retains native architecture AM/UC extracellular matrix maintains quantity activity signals present in fresh AM/UC, high molecular weight hyaluronic acid, heavy chain-HA complex, pentraxin 3. In contrast, were structurally compromised almost completely lacked these crucial components. Conclusion: The results presented here indicate that better preserves signaling foetal Declaration interest: S.C.G. Tseng his family are more than 5% shareholders TissueTech, Inc. its Subsidiaries (Bio-Tissue Amniox Medical) own US Patents Nos. 6,152,142; 6,326,019; PCT/US2010/046675 on method preparation clinical uses human CryoTek distributed by Bio-Tissue, Medical. Tseng, E.K. Tan, H. He employees J. O'Connell M. Cooke C. Mandrycky has no financial conflict. This research was supported a Venture Lab Grant #398 from Georgia Research Alliance, Atlanta, GA, grant Inc., Miami, FL.