Chitosan-microencapsulated rhEGF in promoting wound healing

• DOI: 10.12968/jowc.2021.30.sup9a.ix
• Publication Date: 2021-09-27T17:24:58Z
• Authors: Hsin-Chung Tsai; Christine Sheng; Le-Shin Chang; Zhi-Hong Wen; Ching-Yin Ho; Chuan-Mu Chen
• Keywords: Wound dressing; Occlusive dressing

Chitosan and epidermal growth factor (EGF) have been shown to improve wound healing. This study investigates the healing effects of a spray solution (NewEpi, JoyCom Bio-Chem Co. Ltd., Taiwan) containing recombinant human EGF (rhEGF) delivered via newly patented technology-chitosan microencapsulated nanoparticles.On Wistar rats, two full-thickness wounds on dorsum bilateral spine were created. The rats randomised following treatment groups: hydrogel, wet dressing, foam, rhEGF spray+foam. Sterile dressings applied changed daily. A total 2μg was administered in sprays during each dressing change. All animals euthanised day 14. Tissue samples taken from bed, including an area 2cm surrounding margin for histological evaluations.Wounds treated with achieved greatest size reduction by 14 compared other types conventional dressings. An overall significant difference levels collagen synthesis existed between groups (p<0.01). Pair-wise comparisons showed that significantly promoted higher mature Type I than any (p<0.01), whereas non-rhEGF treatments resulted III collagen. regenerated tissue also alignment normal skin. Epidermis, dermis hair follicles easily observed spray.The major challenge topical application overcome using new drug delivery technology: chitosan-rhEGF nanoparticles. positive this suggest therapeutic potentials novel solution.