New enzymatic-high hydrostatic pressure method to decellularized uterine tissue as scaffold for uterus regeneration in murine model

• DOI: 10.1299/jsmebio.2019.31.2c14
• Publication Date: 2019-06-25T14:15:34Z
• Authors: CHAROENSOMBUT Nar...; Jeong Hyun KIM; Kinyoshi KAWABATA; Tsuyoshi KIMURA; Akio KISHIDA; Takashi USHIDA; Katsuko FURUKAWA; Shu TAKEDA; Takayuki HARADA
• Keywords: Decellularization; Hydrostatic pressure

Infertility is the main obstacle to achieve pregnancy naturally for women. The current technique infertility treatments can not be applied pregnant women who are suffering from uterus malfunction. Tissue engineered organ promising regenerative medicine, which utilizes a scaffold play an important role tissue regeneration. In this research, we studied develop new and efficient method fabricate reconstruction by high hydrostatic pressure decellularization technique. have developed enzymatic-high uterine within 40 minutes. effectively successfully removed cells inside native while preserving natural macro- microstructure.