EXPLOITING NOVEL 3D JELLYFISH COLLAGEN SPONGES FOR BONE TISSUE REGENERATION

• DOI: 10.1302/1358-992x.2023.8.044
• Publication Date: 2023-07-26T13:19:07Z
• Authors: P. Medesan; Y. Chen; P.A. Rust; A. Mearns-Spragg; J.Z. Paxton
• Keywords: Biocompatibility; Bone tissue; Type I collagen; Biomaterial

Jellyfish collagens exhibit auspicious perspectives for tissue engineering applications primarily due to their outstanding compatibility with a wide range of cell types, low immunogenicity and biodegradability. Furthermore, derived from non-mammalian source, jellyfish reduce the risk disease transmission, minimising therefore ethical safety concerns. The current study aims investigate potential 3-dimensional collagen sponges (3D-JCS) in promoting bone regeneration. Both qualitative quantitative analyses were performed order assess adhesion proliferation MC3T3 cells on 3D-JCL, as well migration bone-like ECM production. Histological fluorescent dyes used stain mineral deposits (i.e. Alizarin Red S (ARS), Von Kossa, Tetracycline hydrochloride) while images acquired using optical confocal microscopy. Qualitative data indicated successful 3D-JCS along production both inner outer surface scaffolds. Moreover, four-fold increase ARS uptake between 2- cultures (N=3) an eighteen-fold when cultured osteogenic conditions compared control. This suggests augmented 3D-JCS. Nevertheless, cell-mediated deposition appeared alter mechanical properties that previously reported (compressive modulus: 1-2 kPa before culture). biocompatibility, high porosity pore interconnectivity promoted Their unique features recommend superior biomaterial scaffolds Further studies are required quantify change cell-seeded confirm suitability We predict will be useful future bone-tendon interface Acknowledgments research has been supported by Medical Research Scotland Studentship award (ref: -50177-2019) collaboration Jellagen Ltd.