COMPARISON OF AUTOLOGOUS ADIPOSE-DERIVED TISSUE STROMAL VASCULAR FRACTION (AD-TSVF) BIOMEDICAL INSTRUMENTS FOR THEIR STEM CELL CONTENT

• DOI: 10.1302/1358-992x.2024.1.087
• Publication Date: 2024-01-02T16:12:45Z
• Authors: I. Vargel; M.F. Açil; S.A. Tuncel; N. Baysal; I. Hartuç; H. Okur; F. Korkusuz
• Keywords: Stromal vascular fraction; CD90; CD31

Deriving autologous mesenchymal stem cells (MSCs) from adipose tissues without using enzymes requires sophisticated biomedical instruments. Applied pressure on and are adjusted manually although centrifugation filtration systems frequently used. The number of derived MSCs therefore could differ between We compared the obtained four commercially available devices our newly designed produced instrument (A2, B3, L3, M2 T3). Three-hundred mL tissue was a female patient undergoing liposuction transillumination solution. Obtained equally distributed to each device handled according producers' guides. After handling, 3 stromal vascular fraction (SVF) device. Freshly isolated SVF characterized multi-color flow cytometry (Navios Flow Cytometer, Beckman Coulter, USA). Cell surface antigens were chosen IFATS ISCT. CD31-FITC, CD34-PC5,5, CD73-PE, CD90-PB CD45-A750 (Backman USA) fluorochrome-labeled monoclonal antibodies assessed. Markers combined with ViaKrome (Beckman determine cell viability. At least 10 5 acquired sample. A software EX, used create dot plots calculate composition percentages. data analyzed in Kaluza 2.1 package Graphs prepared GraphPad Prism. CD105 PC7/CD31 FITC percentages 23,9%, 13,5%, 24,6%, 11,4% 28,8% for A2, T3 devices, respectively. conclude that MSC percentage ranged devices. key determinants success orthobiological treatments. Developing should focus increasing while preserving its metabolic activity. Acknowledgments: Scientific Technological Research Council Türkiye (TÜBİTAK)- Technology Innovation Funding Program Directorate (TEYDEB) funded this project (#321893). Servet Kürümoğlu Bariscan Önder Disposet Ltd., Ankara, ( www.disposet.com ) contributed industrial design research studies. Ali Tuncel Feza Korkusuz members Turkish Academy Sciences (TÜBA). Nilsu Baysal by STAR TÜBITAK Grant # 3210893.