Assessment of Bone Marrow Fibrosis and Angiogenesis in Monitoring Patients With Multiple Myeloma

• DOI: 10.1309/ajcpt5y2jriuucub
• Publication Date: 2012-05-14T20:08:06Z
• Authors: Emina Babarović; Toni Valković; Sanja Štifter; Ivana Budisavljević; Irena Seili-Bekafigo; Antica Duletić-Na...; Ksenija Lučin; Nives Jonjić
• Keywords: Aged, 80 and over; Male; 0301 basic medicine; Neovascularization, Pathologic; Biopsy; Plasma Cells; Reproducibility of Results; Antigens, CD34; Kaplan-Meier Estimate; Middle Aged; Prognosis; 3. Good health; 03 medical and health sciences; Bone Marrow; Primary Myelofibrosis; monitoring of multiple myeloma; bone marrow fibrosis; angiogenesis; computerized image analysis; prognosis; Microvessels; Image Processing, Computer-Assisted; Humans; Female; Multiple Myeloma; Aged

The aim of our study was to emphasize the importance of accurate and standardized techniques for detailed monitoring of the microenvironment in multiple myeloma (MM). Bone marrow fibrosis, angiogenesis, and plasma cell infiltrates in bone marrow biopsy (BMB) samples at the time of diagnosis and on completion of therapy were analyzed for 42 patients with newly diagnosed MM. Computerized image analysis was used for all slides stained with anti-CD138 and anti-CD34. The patients with fibrosis in pretreatment BMB samples had significantly higher microvessel density (MVD) and plasma cell infiltrates. In posttreatment BMB samples, nonresponders had a significantly higher frequency and grade of fibrosis and higher values of MVD, total vascular area, and plasma cell percentage. The overall survival of nonresponders and patients with increased marrow fibrosis in posttreatment BMB samples was significantly shorter. The obtained results confirm that complex morphologic examination of the bone marrow microenvironment during the monitoring of MM can provide better prognostic significance.