hAFSC expressing a specific panel of stem cell markers give rise to fully differentiate cardiomyocytes

• DOI: 10.13128/ijae-21801
• Publication Date: 2016-01-01
• Authors: Barbara Ghinassi; Maria Angela D’Amico; Pascal Izzicupo; Simone Guarnieri; Maria A. Mariggiò; Liborio Stuppia; Angela Di Baldass...
• Keywords: Stem cell marker; Multipotent Stem Cell

Human amniotic fluid-derived stem cells (hAFSC) represent a novel class of multipotent sharing characteristics both embryonic and adult cells. In fact, hAFSC proliferate rapidly, are able to differentiate into all the three germ layers, but do not form teratoma. It has been already reported that have cardiac potential, high variability between donors in differentiation efficiency described. Aim this study was phenotypically identify mature cardiomyocytes. hAFSCs from 10 different were characterized for immunophenotypic expression stemness markers then cultured differentiatve conditions. differed efficiency. Only expressing specific cell antigens homogeneous population highly express cytoskeletal proteins structural functional sarcoplasmatic reticulum proteins. Our results demonstrate only showing pattern phenotype can fully myocytes giving rise homogenous by cardiac-specific molecular, structural, properties.