[MicroRNA-107 inhibits the proliferation and invasion of laryngeal squamous cell carcinoma cells by targeting CACNA2D1].

• DOI: 10.13201/j.issn.2096-7993.2020.10.011
• Publication Date: 2020-10-01
• Authors: Chaoping Huang; Yi Wang; Shi Huang; Zhenxiao Wang; Liangfa Liu
• Keywords: clone (Java method); Gentamicin protection assay

Objective:To detect the expression of microRNA-107 （miR-107） and calcium channel protein gene CACNA2D1 in laryngeal cancer tissues, to investigate targeting relationship between miR-107 CACNA2D1, analyze effects on proliferation, invasion colony forming ability cells. Method:Laryngeal tissues normal adjacent tissue samples from 40 patients with were collected, qRT-PCR was used CACNA2D1; Western Blot assay α2δ1 above two tissues; dual-luciferase reporter regulatory effect after overexpression or knockdown human cells TU212 TU686, changes clone formation, detected. Result:The significantly lower than that while just opposite, difference statistically significant （P<0.05）; is higher tissues（P<0.05）; experiments confirmed binds 3'-UTR （202-209, 902-908） mRNA, thereby inhibiting its biological effects; cell showed invasion, formation reduced （P<0.05）, enhanced knocked down （P<0.05） . Conclusion:miR-107 inhibits by CACNA2D1.