Selective Blockade of Voltage-Gated Potassium Channels Reduces Inflammatory Bone Resorption in Experimental Periodontal Disease

0301 basic medicine Antigen Presentation Kv1.3 Potassium Channel CD3 Complex Acid Phosphatase Blotting, Western Alveolar Bone Loss Down-Regulation Gene Expression Cell Differentiation Adoptive Transfer Aggregatibacter actinomycetemcomitans Coculture Techniques 3. Good health Isoenzymes Interferon-gamma 03 medical and health sciences Immunoglobulin G Animals Female Carrier Proteins Bacterial Outer Membrane Proteins Glycoproteins
DOI: 10.1359/jbmr.0301213 Publication Date: 2006-04-27T05:58:24Z
ABSTRACT
The effects of the potassium channel (Kv1.3) blocker kaliotoxin on T-cell-mediated periodontal bone resorption were examined in rats. Systemic administration abrogated conjunction with decreased RANKL mRNA expression by T-cells gingival tissue. This study suggests a plausible therapeutic approach for inflammatory targeting Kv1.3.Kv1.3 is critical to counterbalance calcium influx at T-cell receptor activation. It not known if Kv1.3 also regulates antigen-activated T-cells, and consequently affects vivo mediated activated T-cells.Actinobacillus actinomycetemcomitans 29-kDa outer membrane protein-specific Th1-clone cells used evaluate (using reverse transcriptase-polymerase chain reaction [RT-PCR] Western blot analyses) (0-100 nM) activation parameters ([3H]thymidine incorporation assays ELISA) osteoprotegerin (OPG; flow cytometry, blot, RT-PCR analyses). A rat disease model based adoptive transfer Th1 clone was analyze alveolar OPG T-cells. Stimulated transferred intravenously day 0 all animals (n = 7 per group). Ten micrograms injected subcutaneously twice days 0, 1, 2, 3, after control group rats saline as placebo same injections kaliotoxin-treated group. MOCP-5 osteoclast precursor cell line co-culture studies fixed measure T-cell-derived RANKL-mediated osteoclastogenesis pit formation vitro. Statistical significance evaluated Student's t-test.Kaliotoxin vitro vivo. Most importantly, resulted an 84% decrease induced saline-treated recovered from tissue displayed lower ratios than those ratio protein induction RANKL-dependent markedly treatments vitro.The use or other means block may constitute potential intervention therapy prevent loss disease.
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