Novel Assessment of Bone Using Time-Resolved Transcutaneous Raman Spectroscopy

• DOI: 10.1359/jbmr.050710
• Publication Date: 2006-04-27T07:27:46Z
• Authors: Edward RC Draper; Michael D Morris; Nancy P Camacho; Pavel Matousek; Mike Towrie; Anthony W Parker; Allen E Goodship
• Keywords: Type I collagen

Abstract With fragility fractures increasing as the population ages, there is a need for improved means to estimate risk of fracture. We recorded Raman spectra both mineral and organic phases bone transcutaneously, technology with potential enhance quality fracture assessment. Introduction: The current “gold standard” assessment BMD determined by DXA. However, this accounts only 60–70% strength. X-rays are absorbed phase bone, whereas remains essentially invisible; however, strength critically dependent on phases. report, first time, spectroscopic technique that analyses beneath unbroken skin eliminating spectral components overlying tissues. Materials Methods: used an 800-nm laser (1-kHz, 1-ps pulses) synchronized 4-ps Kerr gate variable picosecond delay effectively shuttered out photons from measured at point 2 mm above carpus two mouse genotypes extreme differences in matrix quality: wildtype oim/oim (matched age, sex, weight). Typical depth was 1.1 mm. repeated measurements tissues removed down bone. Oim/oim mice produce homotrimeric collagen, which results poorly mineralized tissue. Results: main features were present bands similar ratios when through or directly (in genotypes). band (phosphate ν1) smaller skin. associated particular vibrational mode collagen (CH2 wag) showed frequency shift between genotypes. Conclusions: This novel allowed us, make objective transcutaneous bones distinguish normal unhealthy After further optimization, may help improve assessments open opportunities screening anticipation predicted increase fractures.