VEGF Scaffolds Enhance Angiogenesis and Bone Regeneration in Irradiated Osseous Defects

• DOI: 10.1359/jbmr.060120
• Publication Date: 2006-05-30T09:19:38Z
• Authors: Darnell Kaigler; Zhuo Wang; Kim Horger; David J Mooney; Paul H Krebsbach
• Keywords: PLGA

Bone regeneration is challenging in sites where the blood supply has been compromised by radiation. We examined potential of a growth factor (VEGF) delivery system to enhance angiogenesis and bone formation irradiated calvarial defects. VEGF-releasing polymers significantly increased vessel density vascular perfusion defects relative control conditions.Radiation therapy causes damage tissues inhibits its regenerative capacity. Tissue injury from radiation large part caused reduced tissues. The aim this study was determine if endothelial biodegradable PLGA (copolymer D,L-lactide glycolide) scaffold could neovascularization osseous defects.An isolated area calvarium Fisher rats (12 Gy) 2 weeks preoperatively, two 3.5-mm were created area, followed placement scaffolds or VEGF (PLGA with incorporated VEGF) into Laser Doppler imaging performed measure these areas at 1, 2, 6 weeks. Implants retrieved 6, 12 weeks, histologic muCT analyses regeneration.Histological revealed statistically significant increases (>2-fold) function (30%) within compared scaffolds. Additionally, evaluation through histomorphometric greater coverage (26.36 +/- 6.91% versus 7.05 2.09% [SD]) BMD (130.80 58.05 71.28 42.94 mg/cm(3)) scaffolds.Our findings show that have ability defects, outlining novel approach for engineering hypovascular environments.