Morphological Conversion of Calcium Oxalate Crystals Into Stones Is Regulated by Osteopontin in Mouse Kidney

• DOI: 10.1359/jbmr.080514
• Publication Date: 2008-05-27T15:19:48Z
• Authors: Atsushi Okada; Shintaro Nomura; Yukihiko Saeki; Yuji Higashibata; Shuzo Hamamoto; Masahito Hirose; Yasunori Itoh; Takahiro Yasui; Keiichi Tozawa; Kenjiro Kohri
• Keywords: Osteopontin

An important process in kidney stone formation is the conversion of retentive crystals renal tubules to concrete stones. Osteopontin (OPN) major component calcium-containing matrix. In this study, we estimated OPN function early morphological changes calcium oxalate using knockout mice: 100 mg/kg glyoxylate was intra-abdominally injected into wildtype mice (WT) and (KO) for a week, 24-h urine excretion showed no significant difference between WT KO. Kidney crystal depositions were clearly detected by Pizzolato staining but not von Kossa both genotypes, number KO significantly fewer than WT. Morphological observation polarized light optical microphotography scanning electron (SEM) large flower-shaped growing small uniform X-ray diffraction components as monohydrate genotypes. Immunohistochemical that contained protein crystals. We concluded plays crucial role stones mouse kidneys.